PYROGEN TEST
Pyrogen test is defined as a test that detects the presence of bacterial endotoxins (lipopolysaccharides) in a given product or sample including food, air, parenteral […]
Research in Microbiology
World Federation for Culture Collections (WFCC): Sources of pure strains of microorganisms around the world
S/No. Culture Collection Full Name Country/Region 1 AHU AHU Culture Collection Japan 2 ATCC American Type Culture Collection U.S.A. 3 BCRC Bioresource Collection and Research […]
DO SERIAL DILUTION CALCULATIONS WITHOUT STRESS
Links for dilution calculation in biological sciences and other fields of sciences: These links will help you do your dilution calculations without stress. SOLUTION DILUTION […]
DILUTIONS IN MICROBIOLOGY LABORATORY
In the microbiology laboratory, the dilution of fluids, solutions, test samples, antimicrobial agents, microbial cultures or test reagents is amongst the important techniques that are […]
PREPARATION OF ANTIBIOTIC STOCK SOLUTION
Anantibiotic stock solutionis defined as a concentrated solution of an antimicrobial preparation that will be diluted to some lower concentrated solution for actual use. They […]
McFARLAND TURBIDITY STANDARDS
McFarland Turbidity Standard is an important technique performed in the microbiology laboratory especially when carrying out antimicrobial susceptibility studies. It is a turbid solution that […]
STANDARD QUALITY CONTROL STRAINS FOR ANTIBIOGRAM
Quality control strains (or reference strains)are typed cultures of microorganisms with known antimicrobial susceptibility patterns and which are used to ensure consistency, accuracy and reproducibility […]
MINIMUM INHIBITORY CONCENTRATION (MIC): DEFINITION, PROCEDURE & SIGNIFICANCE
Antimicrobial susceptibility tests are used to determine which specific antibiotics or antimicrobial agents a particular pathogenic bacterium or fungus is sensitive to. Susceptibility tests can […]
PREPARATION OF TAE BUFFER
Notes: TAE = tris acetate EDTA buffer TAE Buffer (50X) is a solution used in agarose gel electrophoresis experiments’ and it is typically used for […]
ANTIMICROBIAL SUSCEPTIBILITY TESTING TECHNIQUES
The determination of the antimicrobial susceptibility of a given pathogen to a particular antimicrobial agent or an array of antibiotics can be performed or undertaken […]
GENOTYPIC DETECTION OF ANTIBIOTIC RESISTANT MICROBES
The genotypic detection and characterization of antibiotic resistant genes in pathogenic bacteria is more specific in characterizing resistance genes than the phenotypic detection methods. The […]
VITEK 2 IDENTIFICATION CARD AND OPERATION
Each VITEK 2 identification card has several rows of wells comprising of 8 rows of 8 wells that is made up of 64 wells per […]