STRUCTURE AND COMPONENTS OF GENOMIC DNA
Genomic DNA is a double helix structure that is composed of several components including purines (adenine and guanine) and pyrimidines (cytosine and thymine) as nitrogenous […]
Genetics, Molecular Biology (Genetic Engineering)
Riboswitches and their genetic and molecular significance in living organisms
Riboswitches are defined as RNA domains at the 5’-ends of mRNA that recognize small molecules and respond by changing their three-dimensional structure. This change in […]
GEL ELECTROPHORESIS TECHNIQUE
The term “electrophoresis” refers to the movement of a solid particle (e.g., nucleic acid molecules) through a polymer matrix or gel under the influence of […]
RECOMBINANT DNA TECHNOLOGY (GENETIC ENGINEERING) & APPLICATIONS
Recombinant DNA (rDNA) technology or genetic engineering is the in vitro controlled manipulation of nucleic acids (DNA and RNA) in order to produce new gene […]
Assessing RNA Purity, Concentration and Integrity
The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding to the next stage of the experiment. This is critical […]
RIBONUCLEIC ACID (RNA)
An understanding of the nucleic acid molecules which include deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) is important for us to understand the molecular and/or […]
CLASSIFICATION OF MUTATION BY THEIR EFFECTS ON THE DNA MOLECULE
Based on their effects on the structural integrity of the DNA molecule, mutations can be classified as substitution, insertion, deletion, inversion, reciprocal translocation and chromosomal […]
THE TRANSFORMING PRINCIPLE (FREDRICK GRIFFITH’S EXPERIMENT)
Fredrick Griffith showed in 1928 that deoxyribonucleic acid (DNA) is the genetic material of the cell. His work was unique because it was the first […]
PROTEIN STRUCTURE
Proteins are a group of polypeptides that form a molecule of specific biological function; and they comprises mainly of amino acid sequences that are held […]
PURINES AND PYRIMIDINES
PURINES Purines are heterocyclic aromatic organic compound that consist of a pyrimidine ring fused to an imidazole ring. Purines are the larger of the two […]