STEPS INVOLVED IN TRANSFORMING BACTERIAL CELLS
There are different types of steps involved in the transformation of a bacterial cell in gene cloning techniques. Bacterial cells are transformed when they are […]
Genetics, Molecular Biology (Genetic Engineering)
STEPS INVOLVED IN GENE CLONING
Gene cloning (both cell-based and in vitro technique) involves some series of procedures and these shall be highlighted in this unit. Gene cloning in general […]
GENE CLONING / CLONING
Cloning is a molecular biology technique which is used to make millions of copies of a particular gene of interest or a piece of DNA […]
BLOTTING TECHNIQUE: DEFINITION, TYPES AND PROCEDURE
Blotting is the molecular biology technique that allows molecular biologists to transfer the products of a PCR reaction (particularly nucleic acids and protein molecules) from […]
PCR TECHNIQUE: STEPS AND PROCESSES
PCR TECHNIQUE PCR is the acronym for “polymerase chain reaction”. Polymerase chain reaction is the molecular biology technique that is used to amplify or copy […]
PLASMIDS: definition, application and classification
Plasmids are extrachromosomal DNA molecules found in both eukaryotic and prokaryotic microorganisms; and that have the ability to replicate independently. In prokaryotes, plasmids are found […]
GENOMIC DNA: the main genetic material of the cell
Genomic DNA is the chromosomal DNA of the cell. It is the nucleic acid molecule that directs the reproductive and metabolic activities of the cell. […]
GEL ELECTROPHORESIS TECHNIQUE
The term “electrophoresis” refers to the movement of a solid particle (e.g. nucleic acids) through a polymer matrix or gel under the influence of electric […]
RECOMBINANT DNA TECHNOLOGY (GENETIC ENGINEERING)
Recombinant DNA (rDNA) technology or genetic engineering is the in vitro controlled manipulation of nucleic acids (DNA and RNA) in order to produce new gene […]
FLOW CYTOMETRY TECHNIQUE: IMPORTANCE & APPLICATIONS
Flow cytometry is the high-throughput technique that is used in molecular biology to analyze the chemical and physical properties of samples (especially fluids) when they […]
DNA MICROARRAY: DEFINITION, APPLICATION & USES
DNA microarray is simply a high-throughput molecular biology technique that is used to determine how genes work at any given point in time in a […]
APPLICATIONS AND IMPORTANCE OF ENZYMES USED IN MOLECULAR BIOLOGY EXPERIMENTS
RESTRICTION ENDONUCLEASE: Restriction endonucleases are enzymes that cut nucleic acids (inclusive of DNA and RNA molecules) at specific sites. With restriction enzymes or endonucleases, molecular […]