Sabouraud Dextrose Agar (SDA) Preparation
Sabouraud dextrose agar is a mycological culture media which is used for the selective cultivation and isolation of fungi from both clinical and non-clinical samples. […]
Culture & Culture Media
Calculations for culture media preparation
In this section, you will learn how to calculate for culture media preparation. Calculations for culture media preparation should not be a stressful activity. However, […]
Nutrient agar preparation
Nutrient agar is a general purpose culture media that supports the growth of a handful of bacteria. It is commercially available, and it comes as […]
CHROMAgar – History, Mechanism of action, & Types
Chromogenic media (a.k.a. CHROMAgar) are specialized selective and differential culture media that utilize synthetic chromogenic enzyme substrates in order to specifically target pathogenic species (or […]
DERMATOPHYTE TEST MEDIUM (DTM)
Dermatophyte Test Medium is a modification of a commercial formulation made by Taplin in 1969.It contains soy peptone, dextrose, phenol red, chloramphenicol and cycloheximide. Nitrogenous […]
MacConkey agar
MacConkey agar is both a selective and differential media (Figure 1). By being a selective media, MacConkey agar agar can be used to selectively isolate […]
DESOXYCHOLATE CITRATE AGAR (DCA)
Desoxycholate Citrate Agar (DCA) is a selective medium for the isolation of Salmonella and Shigella organisms. It is ideal for the investigation of pathogenic Enterobacteria. […]
CYSTINE-LACTOSE-ELECTROLYTE-DEFICIENT (CLED) MEDIUM
Cystein-lactose electrolyte deficient (CLED) agar is a differential culture media that is used to isolate uropathogens (i.e., pathogens of the urinary tract system) from urine […]
CHOCOLATE AGAR
Chocolate agar (CA) is used for the selective cultivation and isolation of fastidious organisms (Figure 1). The organisms that can be isolated with chocolate agar […]
CETRIMIDE SELECTIVE AGAR
Cetrimide selective agar is used for the selective cultivation and isolation of Pseudomonas aeruginosa isolates from clinical and non-clinical samples (Figure 1). The preparation of cetrimide selective agar […]