GENERAL GUIDELINE FOR THE PREPARATION OF CULTURE MEDIA IN THE MICROBIOLOGY LABORATORY
Measure out the correct amount of distilled water required for the culture media preparation using a measuring cylinder. The water should be dispensed into a […]
Culture & Culture Media
ISOLATION, CHARACTERIZATION, AND PRESERVATION OF MICROBES
The enrichment techniques for the isolation of microbes from their natural habitat are usually designed for the selective multiplication and/or isolation of only some of […]
PREREQUISITE FOR PREPARATION OF CULTURE MEDIA
Culture media contains several components, some of which are included in already synthesized commercially available growth media, and some of which are included or added […]
TYPES AND CLASSIFICATION OF CULTURE MEDIA
GENERAL PURPOSE MEDIA General purpose media or basic medium is a routine culture media which is used for the cultivation of microorganisms in the microbiology […]
CULTURE (GROWTH) MEDIA: BACTERIOLOGICAL MEDIA
The clinical and laboratory diagnosis of infectious diseases (including those caused by pathogenic bacteria and fungi) in the microbiology laboratory is mainly based on the […]
Sabouraud Dextrose Agar (SDA) Preparation
Sabouraud dextrose agar is a mycological culture media which is used for the selective cultivation and isolation of fungi from both clinical and non-clinical samples. […]
Calculations for culture media preparation
In this section, you will learn how to calculate for culture media preparation. Calculations for culture media preparation should not be a stressful activity. However, […]
Nutrient agar preparation
Nutrient agar is a general purpose culture media that supports the growth of a handful of bacteria. It is commercially available, and it comes as […]
CHROMAgar – History, Mechanism of action, & Types
Chromogenic media (a.k.a. CHROMAgar) are specialized selective and differential culture media that utilize synthetic chromogenic enzyme substrates in order to specifically target pathogenic species (or […]
DERMATOPHYTE TEST MEDIUM (DTM)
Dermatophyte Test Medium is a modification of a commercial formulation made by Taplin in 1969.It contains soy peptone, dextrose, phenol red, chloramphenicol and cycloheximide. Nitrogenous […]
MacConkey agar
MacConkey agar is both a selective and differential media (Figure 1). By being a selective media, MacConkey agar agar can be used to selectively isolate […]
DESOXYCHOLATE CITRATE AGAR (DCA)
Desoxycholate Citrate Agar (DCA) is a selective medium for the isolation of Salmonella and Shigella organisms. It is ideal for the investigation of pathogenic Enterobacteria. […]