CAMP TEST FOR IDENTIFICATION OF GROUP B STREPTOCOCCI
The CAMP test (named for the original authors: Christie, Atkins, and Munch-Petersen) was first used in the identification of group B streptococci (GBS). Group B […]
Biochemical tests
HYDROGEN SULPHIDE TEST
Hydrogen sulphide test is used to identify bacteria that produce the gas, hydrogen sulphide (H2S). Hydrogen sulphide (H2S) production test is used for the detection […]
AMYLASE (STARCH HYDROLYSIS) TEST
Amylase (Starch hydrolysis) test is used to identify bacteria that hydrolyze starch (including amylopectin and amylose) with the help of the enzyme amylase. Amylase is […]
BLOOD AGAR HAEMOLYSIS TEST
Blood agar haemolysis is used to determine the haemolytic ability of some pathogenic microorganisms including Streptococcus pneumoniae, Staphylococcus aureus and Micrococcus species. Some pathogenic bacteria […]
TRIPLE SUGAR IRON AGAR (TSIA)
Triple sugar iron agar (TSIA) is a differential agar medium used to different lactose-fermenting Enterobacteriaceae from non-lactose fermenting enterobacteria. The medium usually contains three sugars […]
CITRATE TEST
Citrate test is used to identify Enterobacteriaceae that utilize citrate or citric acid as their sole carbon and energy source. Simmon’s citrate agar or citrate […]
UREASE TEST
Urease test is used to detect and differentiate Enterobacteriaceae that produce urease enzymes from those that do not. The urease test is used to determine […]
INDOLE TEST
Indole test is used to identify Enterobacteria that breakdown tryptophan (an amino acid) to produce indole. The indole test is a biochemical test performed on […]
OXIDASE TEST
Oxidase test is used to identify microorganisms that produce the enzyme, cytochrome-C oxidase. Cytochrome-C oxidase, a respiratory enzyme is an important enzyme of the electron […]
CATALASE TEST
Catalase test is used to identify microorganisms that produce the enzyme called catalase. It is used to differentiate catalase-producing organisms (e.g. Staphylococcus species) from non-catalase […]
COAGULASE TEST
Coagulase test is used to identify bacteria that produce the enzyme coagulase, and are thus coagulase-positive. It is used to distinguish pathogenic Staphylococcus aureus (which […]
BIOCHEMICAL TESTS USED IN BACTERIOLOGY
Biochemical tests are series of experiments that can be used to differentiate bacteria based on their metabolic activity or ability to utilize a given substrate […]